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Two proteins for the price of one: The design of maximally compressed coding sequences

  • Duke University
  • Stony Brook University

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

The emerging field of synthetic biology moves beyond conventional genetic manipulation to construct novel life forms which do not originate in nature. We explore the problem of designing the provably shortest genomic sequence to encode a given set of genes by exploiting alternate reading frames. We present an algorithm for designing the shortest DNA sequence simultaneously encoding two given amino acid sequences. We show that the coding sequence of naturally occurring pairs of overlapping genes approach maximum compression. We also investigate the impact of alternate coding matrices on overlapping sequence design. Finally, we discuss an interesting application for overlapping gene design, namely the interleaving of an antibiotic resistance gene into a target gene inserted into a virus or plasmid for amplification.

Original languageEnglish
Title of host publicationDNA Computing - 11th International Workshop on DNA Computing, DNA11, Revised Selected Papers
PublisherSpringer Verlag
Pages387-398
Number of pages12
ISBN (Print)3540341617, 9783540341611
DOIs
StatePublished - 2006
Event11th International Workshop on DNA Computing, DNA11 - London, ON, Canada
Duration: Jun 6 2005Jun 9 2005

Publication series

NameLecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
Volume3892 LNCS
ISSN (Print)0302-9743
ISSN (Electronic)1611-3349

Conference

Conference11th International Workshop on DNA Computing, DNA11
Country/TerritoryCanada
CityLondon, ON
Period06/6/0506/9/05

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