Use of silicon and gaas pixel detectors for digital autoradiography

S. R. Amendolia; R. Beccherle; E. Bertolucci; M. G. Bisogni; U. Bottigli; M. Campbell; E. Chesi; M. A. Ciocci; M. Conti; C. Da Via'; A. Del Guerra; S. D'Auria; M. E. Fantacci; M. Gambaccini; G. Grossi; E. Heijne; E. Mancini; R. Marchesini; P. Middelkamp; V. O'Shea; P. Randaccio; N. Romeo; V. Rosso; P. Russo; L. Scharfetter; K. Smith; W. Snoeys; A. Stefanini

doi:10.1109/23.603779

Use of silicon and gaas pixel detectors for digital autoradiography

S. R. Amendolia
, R. Beccherle
, E. Bertolucci
, M. G. Bisogni
, U. Bottigli
, M. Campbell
, E. Chesi
, M. A. Ciocci
, M. Conti
, C. Da Via'
, A. Del Guerra
, S. D'Auria
, M. E. Fantacci
, M. Gambaccini
, G. Grossi
, E. Heijne
, E. Mancini
, R. Marchesini
, P. Middelkamp
, V. O'Shea

P. Randaccio, N. Romeo, V. Rosso, P. Russo, L. Scharfetter, K. Smith, W. Snoeys, A. Stefanini

Physics and Astronomy

University of Sassari
University of Padua
University of Naples Federico II
University of Pisa
CERN
University of Ferrara
University of Glasgow
University of Cagliari

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Solid state detectors made of Si (4.8×8 mm²) and GaAs (6.4×8 mm²) pixel matrices bump-bonded to the Omega2 and Omega3 electronic read-out systems, developed at CERN for H.E.P. experiments, have been used to obtain autoradiographic images of clusters of human epithelial cells and DNA fragments separated via electrophoresis, both labeled with ³²P. The system has shown a good minimum detectable activity per unit area of 2. 10^-4 cps mm^-2, and has proved linear for count rate in the range 0.2-20 cps, typical of autoradiography. The pixel dimensions are 75×500 μm² (SiOmega2) and 50×500 μm² (GaAs-Omega3), respectively. We are able to clearly localize clusters of cells which have incorporated the radioactive tracer and DNA fragments on an electrophoretic gel on paper (blots).

Original language	English
Pages (from-to)	929-933
Number of pages	5
Journal	IEEE Transactions on Nuclear Science
Volume	44
Issue number	3 PART 1
DOIs	https://doi.org/10.1109/23.603779
State	Published - 1997

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Amendolia, S. R., Beccherle, R., Bertolucci, E., Bisogni, M. G., Bottigli, U., Campbell, M., Chesi, E., Ciocci, M. A., Conti, M., Da Via', C., Del Guerra, A., D'Auria, S., Fantacci, M. E., Gambaccini, M., Grossi, G., Heijne, E., Mancini, E., Marchesini, R., Middelkamp, P., ... Stefanini, A. (1997). Use of silicon and gaas pixel detectors for digital autoradiography. IEEE Transactions on Nuclear Science, 44(3 PART 1), 929-933. https://doi.org/10.1109/23.603779

@article{4109a7cd96e14de5bcd010bff2f11918,

title = "Use of silicon and gaas pixel detectors for digital autoradiography",

abstract = "Solid state detectors made of Si (4.8×8 mm2) and GaAs (6.4×8 mm2) pixel matrices bump-bonded to the Omega2 and Omega3 electronic read-out systems, developed at CERN for H.E.P. experiments, have been used to obtain autoradiographic images of clusters of human epithelial cells and DNA fragments separated via electrophoresis, both labeled with 32P. The system has shown a good minimum detectable activity per unit area of 2. 10-4 cps mm-2, and has proved linear for count rate in the range 0.2-20 cps, typical of autoradiography. The pixel dimensions are 75×500 μm2 (SiOmega2) and 50×500 μm2 (GaAs-Omega3), respectively. We are able to clearly localize clusters of cells which have incorporated the radioactive tracer and DNA fragments on an electrophoretic gel on paper (blots).",

author = "Amendolia, \{S. R.\} and R. Beccherle and E. Bertolucci and Bisogni, \{M. G.\} and U. Bottigli and M. Campbell and E. Chesi and Ciocci, \{M. A.\} and M. Conti and \{Da Via'\}, C. and \{Del Guerra\}, A. and S. D'Auria and Fantacci, \{M. E.\} and M. Gambaccini and G. Grossi and E. Heijne and E. Mancini and R. Marchesini and P. Middelkamp and V. O'Shea and P. Randaccio and N. Romeo and V. Rosso and P. Russo and L. Scharfetter and K. Smith and W. Snoeys and A. Stefanini",

year = "1997",

doi = "10.1109/23.603779",

language = "English",

volume = "44",

pages = "929--933",

journal = "IEEE Transactions on Nuclear Science",

issn = "0018-9499",

number = "3 PART 1",

}

Amendolia, SR, Beccherle, R, Bertolucci, E, Bisogni, MG, Bottigli, U, Campbell, M, Chesi, E, Ciocci, MA, Conti, M, Da Via', C, Del Guerra, A, D'Auria, S, Fantacci, ME, Gambaccini, M, Grossi, G, Heijne, E, Mancini, E, Marchesini, R, Middelkamp, P, O'Shea, V, Randaccio, P, Romeo, N, Rosso, V, Russo, P, Scharfetter, L, Smith, K, Snoeys, W & Stefanini, A 1997, 'Use of silicon and gaas pixel detectors for digital autoradiography', IEEE Transactions on Nuclear Science, vol. 44, no. 3 PART 1, pp. 929-933. https://doi.org/10.1109/23.603779

TY - JOUR

T1 - Use of silicon and gaas pixel detectors for digital autoradiography

AU - Amendolia, S. R.

AU - Beccherle, R.

AU - Bertolucci, E.

AU - Bisogni, M. G.

AU - Bottigli, U.

AU - Campbell, M.

AU - Chesi, E.

AU - Ciocci, M. A.

AU - Conti, M.

AU - Da Via', C.

AU - Del Guerra, A.

AU - D'Auria, S.

AU - Fantacci, M. E.

AU - Gambaccini, M.

AU - Grossi, G.

AU - Heijne, E.

AU - Mancini, E.

AU - Marchesini, R.

AU - Middelkamp, P.

AU - O'Shea, V.

AU - Randaccio, P.

AU - Romeo, N.

AU - Rosso, V.

AU - Russo, P.

AU - Scharfetter, L.

AU - Smith, K.

AU - Snoeys, W.

AU - Stefanini, A.

PY - 1997

Y1 - 1997

N2 - Solid state detectors made of Si (4.8×8 mm2) and GaAs (6.4×8 mm2) pixel matrices bump-bonded to the Omega2 and Omega3 electronic read-out systems, developed at CERN for H.E.P. experiments, have been used to obtain autoradiographic images of clusters of human epithelial cells and DNA fragments separated via electrophoresis, both labeled with 32P. The system has shown a good minimum detectable activity per unit area of 2. 10-4 cps mm-2, and has proved linear for count rate in the range 0.2-20 cps, typical of autoradiography. The pixel dimensions are 75×500 μm2 (SiOmega2) and 50×500 μm2 (GaAs-Omega3), respectively. We are able to clearly localize clusters of cells which have incorporated the radioactive tracer and DNA fragments on an electrophoretic gel on paper (blots).

AB - Solid state detectors made of Si (4.8×8 mm2) and GaAs (6.4×8 mm2) pixel matrices bump-bonded to the Omega2 and Omega3 electronic read-out systems, developed at CERN for H.E.P. experiments, have been used to obtain autoradiographic images of clusters of human epithelial cells and DNA fragments separated via electrophoresis, both labeled with 32P. The system has shown a good minimum detectable activity per unit area of 2. 10-4 cps mm-2, and has proved linear for count rate in the range 0.2-20 cps, typical of autoradiography. The pixel dimensions are 75×500 μm2 (SiOmega2) and 50×500 μm2 (GaAs-Omega3), respectively. We are able to clearly localize clusters of cells which have incorporated the radioactive tracer and DNA fragments on an electrophoretic gel on paper (blots).

UR - https://www.scopus.com/pages/publications/33747725936

U2 - 10.1109/23.603779

DO - 10.1109/23.603779

M3 - Article

AN - SCOPUS:33747725936

SN - 0018-9499

VL - 44

SP - 929

EP - 933

JO - IEEE Transactions on Nuclear Science

JF - IEEE Transactions on Nuclear Science

IS - 3 PART 1

ER -

Use of silicon and gaas pixel detectors for digital autoradiography

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