TY - CHAP
T1 - Vaccine Strategies for Cryptococcus neoformans
AU - Brauer, Veronica Soares
AU - Querobino, Samyr Machado
AU - Matos, Gabriel Soares
AU - Dasilva, Deveney
AU - Del Poeta, Maurizio
N1 - Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.
PY - 2024
Y1 - 2024
N2 - Cryptococcus neoformans infections are a major worldwide concern as current treatment strategies are becoming less effective in alleviating the infection. The most extreme and fatal cases are those of immunocompromised individuals. Clinical treatments for cryptococcosis are limited to a few classes of approved drugs, and due to a rise in drug resistance, these drugs are becoming less effective. Therefore, it is essential to develop innovative ways to control this infection. Vaccinations have emerged as a safe, viable, and cost-effective solution to treat a number of diseases over the years. Currently, there are no clinically available vaccines to treat cryptococcal infections, but a number of studies have shown promising results in animal models. Here, we present step-by-step experimental protocols using live-attenuated or heat-killed C. neoformans cells as a vaccination strategy in a preventive or in a therapeutic murine model of cryptococcosis.
AB - Cryptococcus neoformans infections are a major worldwide concern as current treatment strategies are becoming less effective in alleviating the infection. The most extreme and fatal cases are those of immunocompromised individuals. Clinical treatments for cryptococcosis are limited to a few classes of approved drugs, and due to a rise in drug resistance, these drugs are becoming less effective. Therefore, it is essential to develop innovative ways to control this infection. Vaccinations have emerged as a safe, viable, and cost-effective solution to treat a number of diseases over the years. Currently, there are no clinically available vaccines to treat cryptococcal infections, but a number of studies have shown promising results in animal models. Here, we present step-by-step experimental protocols using live-attenuated or heat-killed C. neoformans cells as a vaccination strategy in a preventive or in a therapeutic murine model of cryptococcosis.
KW - CD4 T cells
KW - Cryptococcus neoformans
KW - Heat-killed inactivation
KW - Intramuscular administration
KW - Intranasal administration
KW - Preventive model
KW - T cell depletion
KW - Therapeutic model
KW - Vaccines
UR - https://www.scopus.com/pages/publications/85193543034
U2 - 10.1007/978-1-0716-3722-7_28
DO - 10.1007/978-1-0716-3722-7_28
M3 - Chapter
C2 - 38758334
AN - SCOPUS:85193543034
T3 - Methods in Molecular Biology
SP - 411
EP - 422
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -