TY - GEN
T1 - Volume visualization in cell biology
AU - Kaufman, A.
AU - Yagel, R.
AU - Bakalash, R.
AU - Spector, I.
PY - 1990
Y1 - 1990
N2 - The authors discuss the special properties of volumetric cell data (e.g., noise, discontinuity, raggedness) and the particular difficulties encountered when trying to visualize them in three dimensions. The authors describe some of the solutions adopted, specifically in surface discrimination and shading. Nerve cells (neuroblastoma) grown in tissue culture were selected as the biological preparation because these cells possess very rich actin structures. The cells were stained with a fluorescent probe specific for actin (rhodamine-phalloidin) and were viewed and optically sectioned using the Bio-Rad MRC 600 confocal fluorescence microscope. The slice dataset was then reconstructed and processed in the BioCube environment, a comprehensive system developed for volume visualization of cellular structures. The actin cytoskeleton of single cells was visualized and manipulated using this system.
AB - The authors discuss the special properties of volumetric cell data (e.g., noise, discontinuity, raggedness) and the particular difficulties encountered when trying to visualize them in three dimensions. The authors describe some of the solutions adopted, specifically in surface discrimination and shading. Nerve cells (neuroblastoma) grown in tissue culture were selected as the biological preparation because these cells possess very rich actin structures. The cells were stained with a fluorescent probe specific for actin (rhodamine-phalloidin) and were viewed and optically sectioned using the Bio-Rad MRC 600 confocal fluorescence microscope. The slice dataset was then reconstructed and processed in the BioCube environment, a comprehensive system developed for volume visualization of cellular structures. The actin cytoskeleton of single cells was visualized and manipulated using this system.
UR - https://www.scopus.com/pages/publications/0025541790
M3 - Conference contribution
AN - SCOPUS:0025541790
SN - 0818620838
T3 - Proc First 90 IEEE Conf Visualization Visualization 90
SP - 160-168, 47
BT - Proc First 90 IEEE Conf Visualization Visualization 90
PB - Publ by IEEE
T2 - Proceedings of the First 1990 IEEE Conference on Visualization - Visualization '90
Y2 - 23 October 1990 through 26 October 1990
ER -