Widefield Fluorescence Optical Sectioning Microscopy in a Miniature Fiber-Coupled Microscope with Active Axial Scanning

Gabriel Martinez Sanchez; Omkar D. Supekar; Gregory L. Futia; Baris N. Ozbay; Cristin Welle; Victor M. Bright; Juliet T. Gopinath; DIego Restrepo; Douglas Shepherd; Emily A. Gibson

Widefield Fluorescence Optical Sectioning Microscopy in a Miniature Fiber-Coupled Microscope with Active Axial Scanning

Gabriel Martinez Sanchez
, Omkar D. Supekar
, Gregory L. Futia
, Baris N. Ozbay
, Cristin Welle
, Victor M. Bright
, Juliet T. Gopinath
, DIego Restrepo
, Douglas Shepherd
, Emily A. Gibson

Physiology and Biophysics

University of Colorado Denver
University of Colorado Boulder
University of Colorado Anschutz Medical Campus
Arizona State University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

We present widefield structured illumination in a miniature, light-weight fiber-coupled microscope with electrowetting axial scanning. We demonstrate imaging of YFP-labeled neurons in mouse brain tissue showing potential for fast volumetric imaging in freely moving animals.

Original language	English
Title of host publication	2020 Conference on Lasers and Electro-Optics, CLEO 2020 - Proceedings
Publisher	Institute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)	9781943580767
State	Published - May 2020
Event	2020 Conference on Lasers and Electro-Optics, CLEO 2020 - San Jose, United States Duration: May 10 2020 → May 15 2020

Publication series

Name	Conference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS
Volume	2020-May
ISSN (Print)	1092-8081

Conference

Conference	2020 Conference on Lasers and Electro-Optics, CLEO 2020
Country/Territory	United States
City	San Jose
Period	05/10/20 → 05/15/20

Cite this

Sanchez, G. M., Supekar, O. D., Futia, G. L., Ozbay, B. N., Welle, C., Bright, V. M., Gopinath, J. T., Restrepo, DI., Shepherd, D., & Gibson, E. A. (2020). Widefield Fluorescence Optical Sectioning Microscopy in a Miniature Fiber-Coupled Microscope with Active Axial Scanning. In 2020 Conference on Lasers and Electro-Optics, CLEO 2020 - Proceedings Article 9193149 (Conference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS; Vol. 2020-May). Institute of Electrical and Electronics Engineers Inc..

Sanchez, Gabriel Martinez ; Supekar, Omkar D. ; Futia, Gregory L. et al. / Widefield Fluorescence Optical Sectioning Microscopy in a Miniature Fiber-Coupled Microscope with Active Axial Scanning. 2020 Conference on Lasers and Electro-Optics, CLEO 2020 - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2020. (Conference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS).

@inproceedings{3fa09964cfaa409baac5d2a54d330ebf,

title = "Widefield Fluorescence Optical Sectioning Microscopy in a Miniature Fiber-Coupled Microscope with Active Axial Scanning",

abstract = "We present widefield structured illumination in a miniature, light-weight fiber-coupled microscope with electrowetting axial scanning. We demonstrate imaging of YFP-labeled neurons in mouse brain tissue showing potential for fast volumetric imaging in freely moving animals.",

author = "Sanchez, \{Gabriel Martinez\} and Supekar, \{Omkar D.\} and Futia, \{Gregory L.\} and Ozbay, \{Baris N.\} and Cristin Welle and Bright, \{Victor M.\} and Gopinath, \{Juliet T.\} and DIego Restrepo and Douglas Shepherd and Gibson, \{Emily A.\}",

note = "Publisher Copyright: {\textcopyright} 2020 OSA.; 2020 Conference on Lasers and Electro-Optics, CLEO 2020 ; Conference date: 10-05-2020 Through 15-05-2020",

year = "2020",

month = may,

language = "English",

series = "Conference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

booktitle = "2020 Conference on Lasers and Electro-Optics, CLEO 2020 - Proceedings",

}

Sanchez, GM, Supekar, OD, Futia, GL, Ozbay, BN, Welle, C, Bright, VM, Gopinath, JT, Restrepo, DI, Shepherd, D & Gibson, EA 2020, Widefield Fluorescence Optical Sectioning Microscopy in a Miniature Fiber-Coupled Microscope with Active Axial Scanning. in 2020 Conference on Lasers and Electro-Optics, CLEO 2020 - Proceedings., 9193149, Conference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS, vol. 2020-May, Institute of Electrical and Electronics Engineers Inc., 2020 Conference on Lasers and Electro-Optics, CLEO 2020, San Jose, United States, 05/10/20.

Widefield Fluorescence Optical Sectioning Microscopy in a Miniature Fiber-Coupled Microscope with Active Axial Scanning. / Sanchez, Gabriel Martinez; Supekar, Omkar D.; Futia, Gregory L. et al.
2020 Conference on Lasers and Electro-Optics, CLEO 2020 - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2020. 9193149 (Conference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS; Vol. 2020-May).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Widefield Fluorescence Optical Sectioning Microscopy in a Miniature Fiber-Coupled Microscope with Active Axial Scanning

AU - Sanchez, Gabriel Martinez

AU - Supekar, Omkar D.

AU - Futia, Gregory L.

AU - Ozbay, Baris N.

AU - Welle, Cristin

AU - Bright, Victor M.

AU - Gopinath, Juliet T.

AU - Restrepo, DIego

AU - Shepherd, Douglas

AU - Gibson, Emily A.

PY - 2020/5

Y1 - 2020/5

N2 - We present widefield structured illumination in a miniature, light-weight fiber-coupled microscope with electrowetting axial scanning. We demonstrate imaging of YFP-labeled neurons in mouse brain tissue showing potential for fast volumetric imaging in freely moving animals.

AB - We present widefield structured illumination in a miniature, light-weight fiber-coupled microscope with electrowetting axial scanning. We demonstrate imaging of YFP-labeled neurons in mouse brain tissue showing potential for fast volumetric imaging in freely moving animals.

UR - https://www.scopus.com/pages/publications/85091635348

M3 - Conference contribution

AN - SCOPUS:85091635348

T3 - Conference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS

BT - 2020 Conference on Lasers and Electro-Optics, CLEO 2020 - Proceedings

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 2020 Conference on Lasers and Electro-Optics, CLEO 2020

Y2 - 10 May 2020 through 15 May 2020

ER -

Sanchez GM, Supekar OD, Futia GL, Ozbay BN, Welle C, Bright VM et al. Widefield Fluorescence Optical Sectioning Microscopy in a Miniature Fiber-Coupled Microscope with Active Axial Scanning. In 2020 Conference on Lasers and Electro-Optics, CLEO 2020 - Proceedings. Institute of Electrical and Electronics Engineers Inc. 2020. 9193149. (Conference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS).

Widefield Fluorescence Optical Sectioning Microscopy in a Miniature Fiber-Coupled Microscope with Active Axial Scanning

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