Widefield fluorescence optical sectioning microscopy in a miniature fiber-coupled microscope with active axial scanning

Gabriel Martinez Sanchez; Omkar D. Supekar; Gregory L. Futia; Baris N. Ozbay; Cristin Welle; Victor M. Bright; Juliet T. Gopinath; Diego Restrepo; Douglas Shepherd; Emily A. Gibson

doi:10.1364/CLEO_SI.2020.SW4P.4

Widefield fluorescence optical sectioning microscopy in a miniature fiber-coupled microscope with active axial scanning

Gabriel Martinez Sanchez
, Omkar D. Supekar
, Gregory L. Futia
, Baris N. Ozbay
, Cristin Welle
, Victor M. Bright
, Juliet T. Gopinath
, Diego Restrepo
, Douglas Shepherd
, Emily A. Gibson

Physiology and Biophysics

Department of Bioengineering
Department of Mechanical Engineering
University of Colorado Anschutz Medical Campus
University of Colorado Boulder
Arizona State University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

We present widefield structured illumination in a miniature, light-weight fiber-coupled microscope with electrowetting axial scanning. We demonstrate imaging of YFP-labeled neurons in mouse brain tissue showing potential for fast volumetric imaging in freely moving animals.

Original language	English
Title of host publication	CLEO
Subtitle of host publication	Science and Innovations, CLEO_SI 2020
Publisher	Optica Publishing Group (formerly OSA)
ISBN (Print)	9781943580767
DOIs	https://doi.org/10.1364/CLEO_SI.2020.SW4P.4
State	Published - 2020
Event	CLEO: Science and Innovations, CLEO_SI 2020 - Washington, United States Duration: May 10 2020 → May 15 2020

Publication series

Name	Optics InfoBase Conference Papers
Volume	Part F183-CLEO-SI 2020
ISSN (Electronic)	2162-2701

Conference

Conference	CLEO: Science and Innovations, CLEO_SI 2020
Country/Territory	United States
City	Washington
Period	05/10/20 → 05/15/20

Access to Document

10.1364/CLEO_SI.2020.SW4P.4

Cite this

Sanchez, G. M., Supekar, O. D., Futia, G. L., Ozbay, B. N., Welle, C., Bright, V. M., Gopinath, J. T., Restrepo, D., Shepherd, D., & Gibson, E. A. (2020). Widefield fluorescence optical sectioning microscopy in a miniature fiber-coupled microscope with active axial scanning. In CLEO: Science and Innovations, CLEO_SI 2020 Article SW4P.4 (Optics InfoBase Conference Papers; Vol. Part F183-CLEO-SI 2020). Optica Publishing Group (formerly OSA). https://doi.org/10.1364/CLEO_SI.2020.SW4P.4

@inproceedings{e22656f81c904e40afcd2f61512b3cc8,

title = "Widefield fluorescence optical sectioning microscopy in a miniature fiber-coupled microscope with active axial scanning",

abstract = "We present widefield structured illumination in a miniature, light-weight fiber-coupled microscope with electrowetting axial scanning. We demonstrate imaging of YFP-labeled neurons in mouse brain tissue showing potential for fast volumetric imaging in freely moving animals.",

author = "Sanchez, \{Gabriel Martinez\} and Supekar, \{Omkar D.\} and Futia, \{Gregory L.\} and Ozbay, \{Baris N.\} and Cristin Welle and Bright, \{Victor M.\} and Gopinath, \{Juliet T.\} and Diego Restrepo and Douglas Shepherd and Gibson, \{Emily A.\}",

note = "Publisher Copyright: {\textcopyright} OSA 2020 {\textcopyright} 2020 The Author(s); CLEO: Science and Innovations, CLEO\_SI 2020 ; Conference date: 10-05-2020 Through 15-05-2020",

year = "2020",

doi = "10.1364/CLEO\_SI.2020.SW4P.4",

language = "English",

isbn = "9781943580767",

series = "Optics InfoBase Conference Papers",

publisher = "Optica Publishing Group (formerly OSA)",

booktitle = "CLEO",

}

Sanchez, GM, Supekar, OD, Futia, GL, Ozbay, BN, Welle, C, Bright, VM, Gopinath, JT, Restrepo, D, Shepherd, D & Gibson, EA 2020, Widefield fluorescence optical sectioning microscopy in a miniature fiber-coupled microscope with active axial scanning. in CLEO: Science and Innovations, CLEO_SI 2020., SW4P.4, Optics InfoBase Conference Papers, vol. Part F183-CLEO-SI 2020, Optica Publishing Group (formerly OSA), CLEO: Science and Innovations, CLEO_SI 2020, Washington, United States, 05/10/20. https://doi.org/10.1364/CLEO_SI.2020.SW4P.4

Widefield fluorescence optical sectioning microscopy in a miniature fiber-coupled microscope with active axial scanning. / Sanchez, Gabriel Martinez; Supekar, Omkar D.; Futia, Gregory L. et al.
CLEO: Science and Innovations, CLEO_SI 2020. Optica Publishing Group (formerly OSA), 2020. SW4P.4 (Optics InfoBase Conference Papers; Vol. Part F183-CLEO-SI 2020).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Widefield fluorescence optical sectioning microscopy in a miniature fiber-coupled microscope with active axial scanning

AU - Sanchez, Gabriel Martinez

AU - Supekar, Omkar D.

AU - Futia, Gregory L.

AU - Ozbay, Baris N.

AU - Welle, Cristin

AU - Bright, Victor M.

AU - Gopinath, Juliet T.

AU - Restrepo, Diego

AU - Shepherd, Douglas

AU - Gibson, Emily A.

PY - 2020

Y1 - 2020

N2 - We present widefield structured illumination in a miniature, light-weight fiber-coupled microscope with electrowetting axial scanning. We demonstrate imaging of YFP-labeled neurons in mouse brain tissue showing potential for fast volumetric imaging in freely moving animals.

AB - We present widefield structured illumination in a miniature, light-weight fiber-coupled microscope with electrowetting axial scanning. We demonstrate imaging of YFP-labeled neurons in mouse brain tissue showing potential for fast volumetric imaging in freely moving animals.

UR - https://www.scopus.com/pages/publications/85095437573

U2 - 10.1364/CLEO_SI.2020.SW4P.4

DO - 10.1364/CLEO_SI.2020.SW4P.4

M3 - Conference contribution

AN - SCOPUS:85095437573

SN - 9781943580767

T3 - Optics InfoBase Conference Papers

BT - CLEO

PB - Optica Publishing Group (formerly OSA)

T2 - CLEO: Science and Innovations, CLEO_SI 2020

Y2 - 10 May 2020 through 15 May 2020

ER -

Widefield fluorescence optical sectioning microscopy in a miniature fiber-coupled microscope with active axial scanning

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